%0 Figure %A Sepako, Enoch %A J. Glennie, Sarah %A C. Jambo, Kondwani %A Mzinza, David %A H. Iwajomo, Oluwadamilola %A Banda, Dominic %A J. van Oosterhout, Joep %A A. Williams, Neil %A B. Gordon, Stephen %A S. Heyderman, Robert %D 2014 %T Pneumococcal-specific CD4+ T-cell responses in peripheral blood during ART. %U https://plos.figshare.com/articles/figure/_Pneumococcal_specific_CD4_T_cell_responses_in_peripheral_blood_during_ART_/1082170 %R 10.1371/journal.pone.0100640.g002 %2 https://plos.figshare.com/ndownloader/files/1563413 %K immunology %K Immune system %K Acquired immune system %K Immune response %K immunity %K microbiology %K Medical microbiology %K Microbial pathogens %K Bacterial pathogens %K streptococcus %K Viral pathogens %K Immunodeficiency viruses %K Virology %K t-cell %K responses %K peripheral %X

Patients were analysed prior to initiation of ART and followed-up at 3, 6 and 12 mths ART for CD4+ T-cell responses. Responses from HIV-infected persons at 12 months ART were compared with HIV-negative persons recruited from the same community (A) ex vivo IFN-γ ELISpot responses to wild-type Streptococcus pneumoniae strain concentrated culture supernatant (CCS) (D39WT CCS) (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 21) (B) ex vivo IFN-γ ELISpot responses to an isogenic pneumolysin (ply)-deficient mutant (D39Ply- CCS) (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 21) (C) proliferative responses (8 day CFSE dilution assay) to D39WT CCS (0mth, n = 16; 6mths, n = 13; 12mths, n = 13; HIV-, 28) (D) proliferative responses to D39Ply- CCS (0mth, n = 17; 6mths, n = 14; 12mths, n = 13; HIV-, 28) (E) CD154 expression on activated CD4+ T cells in response to D39WT CCS (0mth, n = 33; 3mths, n = 30; 6mths, n = 30; 12mths, n = 16; HIV-, 29) (F) CD154 expression on activated CD4+ T cells in response to D39Ply- CCS (0mth, n = 30; 3mths, n = 29; 6mths, n = 28; 12mths, n = 14; HIV-, 29). Black horizontal bars represent median and IQR after background responses were substrated from all antigen-specific CD4+ T cell responses. Wilcoxon matched pairs were used to compare T-cell characteristics of HIV-infected persons on ART over time, and the Mann Whitney U test in the HIV-infected and HIV-uninfected comparisons. Representative flow cytometric data demonstrating CD4+ T-cell proliferative responses and CD154 expression and gating strategy are shown in Figure S1.

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