10.1371/journal.pone.0100640.g002
Enoch Sepako
Enoch
Sepako
Sarah J. Glennie
Sarah
J. Glennie
Kondwani C. Jambo
Kondwani
C. Jambo
David Mzinza
David
Mzinza
Oluwadamilola H. Iwajomo
Oluwadamilola
H. Iwajomo
Dominic Banda
Dominic
Banda
Joep J. van Oosterhout
Joep
J. van Oosterhout
Neil A. Williams
Neil
A. Williams
Stephen B. Gordon
Stephen
B. Gordon
Robert S. Heyderman
Robert
S. Heyderman
Pneumococcal-specific CD4+ T-cell responses in peripheral blood during ART.
Public Library of Science
2014
immunology
Immune system
Acquired immune system
Immune response
immunity
microbiology
Medical microbiology
Microbial pathogens
Bacterial pathogens
streptococcus
Viral pathogens
Immunodeficiency viruses
Virology
t-cell
responses
peripheral
2014-06-24 14:56:46
Figure
https://plos.figshare.com/articles/figure/_Pneumococcal_specific_CD4_T_cell_responses_in_peripheral_blood_during_ART_/1082170
<p>Patients were analysed prior to initiation of ART and followed-up at 3, 6 and 12 mths ART for CD4<sup>+</sup> T-cell responses. Responses from HIV-infected persons at 12 months ART were compared with HIV-negative persons recruited from the same community (<b>A</b>) <i>ex vivo</i> IFN-γ ELISpot responses to wild-type <i>Streptococcus pneumoniae</i> strain concentrated culture supernatant (CCS) (D39WT CCS) (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 21) (<b>B</b>) <i>ex vivo</i> IFN-γ ELISpot responses to an isogenic pneumolysin (ply)-deficient mutant (D39Ply- CCS) (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 21) (<b>C</b>) proliferative responses (8 day CFSE dilution assay) to D39WT CCS (0mth, n = 16; 6mths, n = 13; 12mths, n = 13; HIV-, 28) (<b>D</b>) proliferative responses to D39Ply- CCS (0mth, n = 17; 6mths, n = 14; 12mths, n = 13; HIV-, 28) (<b>E</b>) CD154 expression on activated CD4<sup>+</sup> T cells in response to D39WT CCS (0mth, n = 33; 3mths, n = 30; 6mths, n = 30; 12mths, n = 16; HIV-, 29) (<b>F</b>) CD154 expression on activated CD4<sup>+</sup> T cells in response to D39Ply- CCS (0mth, n = 30; 3mths, n = 29; 6mths, n = 28; 12mths, n = 14; HIV-, 29). Black horizontal bars represent median and IQR after background responses were substrated from all antigen-specific CD4<sup>+</sup> T cell responses. Wilcoxon matched pairs were used to compare T-cell characteristics of HIV-infected persons on ART over time, and the Mann Whitney U test in the HIV-infected and HIV-uninfected comparisons. Representative flow cytometric data demonstrating CD4<sup>+</sup> T-cell proliferative responses and CD154 expression and gating strategy are shown in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100640#pone.0100640.s001" target="_blank">Figure S1</a></b>.</p>