%0 Figure %A Tian, Zongwen %A Wang, Changhua %A Hu, Chengjun %A Tian, Yihao %A Liu, Jinbao %A Wang, Xuejun %D 2014 %T Autophagic inhibition accumulates GFPu in a p62 dependent manner. %U https://plos.figshare.com/articles/figure/_Autophagic_inhibition_accumulates_GFPu_in_a_p62_dependent_manner_/1081428 %R 10.1371/journal.pone.0100715.g007 %2 https://plos.figshare.com/ndownloader/files/1562446 %K Biochemistry %K proteins %K Ubiquitin-proteasome system %K cell biology %K Cellular structures and organelles %K lysosomes %K cardiology %K inhibition %K accumulates %K gfpu %K p62 %X

Cultured NRVMs were infected with adenoviruses to express GFPu and RFP (Ad-GFPu/RFP) 24 hours before transfection with siRNA for Atg7 (siAtg7), Rab7 (siRab7) and/or for p62 (sip62). A luciferase-specific siRNA (siLuc) was used as control for siRNA infection and off-target effects. The cells were harvested at 48 h later for extracting total proteins. A, Shown are representative images out of 3 repeats. B, pooled densitometry data. *p<0.05 vs. CTL.

%I PLOS ONE