10.1371/journal.pbio.1001895.g006 Cendrine Tourette Cendrine Tourette Francesca Farina Francesca Farina Rafael P. Vazquez-Manrique Rafael P. Vazquez-Manrique Anne-Marie Orfila Anne-Marie Orfila Jessica Voisin Jessica Voisin Sonia Hernandez Sonia Hernandez Nicolas Offner Nicolas Offner J. Alex Parker J. Alex Parker Sophie Menet Sophie Menet Jinho Kim Jinho Kim Jungmok Lyu Jungmok Lyu Si Ho Choi Si Ho Choi Kerry Cormier Kerry Cormier Christina K. Edgerly Christina K. Edgerly Olivia L. Bordiuk Olivia L. Bordiuk Karen Smith Karen Smith Anne Louise Anne Louise Michael Halford Michael Halford Steven Stacker Steven Stacker Jean-Philippe Vert Jean-Philippe Vert Robert J. Ferrante Robert J. Ferrante Wange Lu Wange Lu Christian Neri Christian Neri Reducing presenilin 1 expression counteracts the cytotoxicity of full-length Ryk overexpression in mutant htt striatal cells. Public Library of Science 2014 neuroscience neurology Neurobiology of disease and regeneration Model organisms Animal models presenilin counteracts cytotoxicity full-length ryk overexpression mutant htt striatal 2014-06-24 03:20:54 Figure https://plos.figshare.com/articles/figure/_Reducing_presenilin_1_expression_counteracts_the_cytotoxicity_of_full_length_Ryk_overexpression_in_mutant_htt_striatal_cells_/1081427 <p>Assays were performed using caspase 3/7 activity in cells subjected to serum deprivation. (A) PS1 and PS2 siRNA treatment enhances the viability of mutant htt striatal cells. Data are mean ± SD (<i>n</i> = 3), *<i>p</i><0.01 compared to scramble RNA. Significance was tested using one-way ANOVA, with correction for multiple testing by Tukey's Multiple Comparison Test. (B) Representative Western blots showing decreased levels of PS1/PS2. (C) Knockdown of PS1 reduces the cytotoxic effects of overexpressing full-length Ryk in mutant htt striatal cells, with no effect detected on the cytotoxic effects of overexpressing Ryk-ICD. Data are mean ± SD (<i>n</i> = 4), *<i>p</i><0.05 and **<i>p</i><0.01 compared to scramble RNA. Significance was tested using one-way ANOVA, with correction for multiple testing by Tukey's Multiple Comparison Test. EV, empty vector; ns, not significant. (D) Representative Western blots showing decreased levels of PS1 and expression of Myc-tagged Ryk species and Myc-tagged Ryk-ICD. *Nonspecific signal.</p>