10.1371/journal.pbio.1001895.g006
Cendrine Tourette
Cendrine
Tourette
Francesca Farina
Francesca
Farina
Rafael P. Vazquez-Manrique
Rafael P.
Vazquez-Manrique
Anne-Marie Orfila
Anne-Marie
Orfila
Jessica Voisin
Jessica
Voisin
Sonia Hernandez
Sonia
Hernandez
Nicolas Offner
Nicolas
Offner
J. Alex Parker
J.
Alex Parker
Sophie Menet
Sophie
Menet
Jinho Kim
Jinho
Kim
Jungmok Lyu
Jungmok
Lyu
Si Ho Choi
Si
Ho Choi
Kerry Cormier
Kerry
Cormier
Christina K. Edgerly
Christina
K. Edgerly
Olivia L. Bordiuk
Olivia
L. Bordiuk
Karen Smith
Karen
Smith
Anne Louise
Anne
Louise
Michael Halford
Michael
Halford
Steven Stacker
Steven
Stacker
Jean-Philippe Vert
Jean-Philippe
Vert
Robert J. Ferrante
Robert J.
Ferrante
Wange Lu
Wange
Lu
Christian Neri
Christian
Neri
Reducing presenilin 1 expression counteracts the cytotoxicity of full-length Ryk overexpression in mutant htt striatal cells.
Public Library of Science
2014
neuroscience
neurology
Neurobiology of disease and regeneration
Model organisms
Animal models
presenilin
counteracts
cytotoxicity
full-length
ryk
overexpression
mutant
htt
striatal
2014-06-24 03:20:54
Figure
https://plos.figshare.com/articles/figure/_Reducing_presenilin_1_expression_counteracts_the_cytotoxicity_of_full_length_Ryk_overexpression_in_mutant_htt_striatal_cells_/1081427
<p>Assays were performed using caspase 3/7 activity in cells subjected to serum deprivation. (A) PS1 and PS2 siRNA treatment enhances the viability of mutant htt striatal cells. Data are mean ± SD (<i>n</i> = 3), *<i>p</i><0.01 compared to scramble RNA. Significance was tested using one-way ANOVA, with correction for multiple testing by Tukey's Multiple Comparison Test. (B) Representative Western blots showing decreased levels of PS1/PS2. (C) Knockdown of PS1 reduces the cytotoxic effects of overexpressing full-length Ryk in mutant htt striatal cells, with no effect detected on the cytotoxic effects of overexpressing Ryk-ICD. Data are mean ± SD (<i>n</i> = 4), *<i>p</i><0.05 and **<i>p</i><0.01 compared to scramble RNA. Significance was tested using one-way ANOVA, with correction for multiple testing by Tukey's Multiple Comparison Test. EV, empty vector; ns, not significant. (D) Representative Western blots showing decreased levels of PS1 and expression of Myc-tagged Ryk species and Myc-tagged Ryk-ICD. *Nonspecific signal.</p>