Representative confocal fluorescence micrographs of NRVMs grown on cover glasses.
Zongwen Tian
Changhua Wang
Chengjun Hu
Yihao Tian
Jinbao Liu
Xuejun Wang
10.1371/journal.pone.0100715.g006
https://plos.figshare.com/articles/figure/_Representative_confocal_fluorescence_micrographs_of_NRVMs_grown_on_cover_glasses_/1081426
<p>Ad-GFPu infection and BFA (10 nM) treatment are as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100715#pone-0100715-g004" target="_blank"><b>Figure 4</b></a>. At 24 hr after BFA treatment, the cells were fixed with 4% paraformaldehyde and subject to immunofluorescence staining for p62 (red). GFPu direct fluorescence (green) and p62 indirect immunofluorescence were visualized and captured using a confocal microscope. In the BFA treated cells, both p62 and GFPu are increased and the increased GFPu is co-localized with p62. Scale bar = 50 µm.</p>
2014-06-24 03:20:11
Biochemistry
proteins
Ubiquitin-proteasome system
cell biology
Cellular structures and organelles
lysosomes
cardiology
confocal
fluorescence
micrographs
nrvms
grown