Inhibition of the ALP stabilizes a UPS substrate. Zongwen Tian Changhua Wang Chengjun Hu Yihao Tian Jinbao Liu Xuejun Wang 10.1371/journal.pone.0100715.g005 https://plos.figshare.com/articles/figure/_Inhibition_of_the_ALP_stabilizes_a_UPS_substrate_/1081423 <p>NRVMs were cultured and infected with Ad-GFPu/Ad-RFP viral mixture as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100715#pone-0100715-g004" target="_blank"><b>Figure 4</b></a>. Forty-eight hours later, the cells were treated with 3-MA (2 mM, <b>A</b> and <b>B</b>) or BFA (10 nM, <b>C</b> and <b>D</b>), or respective vehicle control (CTL) for 12 hrs; then, the treatment of cycloheximide (CHX, 50 µM) was initiated. Immediately before (0 min) or 5, 10, 15, 30, 60, and 120 min after CHX administration, the cells were harvested for western blot analyses for GFPu and RFP. Representative western blot analysis images (<b>A</b>, <b>C</b>) and densitometry data pooled from 3 biological repeats (<b>B</b>, <b>D</b>) are shown. *<i>p</i><0.05, **<i>p</i><0.01 vs. CTL.</p> 2014-06-24 03:20:11 Biochemistry proteins Ubiquitin-proteasome system cell biology Cellular structures and organelles lysosomes cardiology alp stabilizes ups