Inhibition of the ALP stabilizes a UPS substrate.
Zongwen Tian
Changhua Wang
Chengjun Hu
Yihao Tian
Jinbao Liu
Xuejun Wang
10.1371/journal.pone.0100715.g005
https://plos.figshare.com/articles/figure/_Inhibition_of_the_ALP_stabilizes_a_UPS_substrate_/1081423
<p>NRVMs were cultured and infected with Ad-GFPu/Ad-RFP viral mixture as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100715#pone-0100715-g004" target="_blank"><b>Figure 4</b></a>. Forty-eight hours later, the cells were treated with 3-MA (2 mM, <b>A</b> and <b>B</b>) or BFA (10 nM, <b>C</b> and <b>D</b>), or respective vehicle control (CTL) for 12 hrs; then, the treatment of cycloheximide (CHX, 50 µM) was initiated. Immediately before (0 min) or 5, 10, 15, 30, 60, and 120 min after CHX administration, the cells were harvested for western blot analyses for GFPu and RFP. Representative western blot analysis images (<b>A</b>, <b>C</b>) and densitometry data pooled from 3 biological repeats (<b>B</b>, <b>D</b>) are shown. *<i>p</i><0.05, **<i>p</i><0.01 vs. CTL.</p>
2014-06-24 03:20:11
Biochemistry
proteins
Ubiquitin-proteasome system
cell biology
Cellular structures and organelles
lysosomes
cardiology
alp
stabilizes
ups