Mutational analysis of LASV L endonuclease domain in the LASV minigenomic RNA transcription assay.
Gregor D. Wallat
Qinfeng Huang
Wenjian Wang
Haohao Dong
Hinh Ly
Yuying Liang
Changjiang Dong
10.1371/journal.pone.0087577.g004
https://plos.figshare.com/articles/figure/_Mutational_analysis_of_LASV_L_endonuclease_domain_in_the_LASV_minigenomic_RNA_transcription_assay_/1080186
<p>FLAG-tagged L expression vector with either WT or alanine substitution at the respective residue within the N-terminal endonuclease domain was used to transfect 293T cells, together with the myc-tagged NP expression vector and LUC-encoded LASV MG RNA. LUC activity was measured and plotted in log scale. Results shown are the average of at least three independent experiments with error bars representing standard deviations. The expression of L (WT or mutant), NP, and GAPDH in the transfected cells was detected by Western blot analysis using anti-FLAG, anti-myc, and anti-GAPDH antibody, respectively.</p>
2014-02-07 11:36:37
Biochemistry
proteins
Protein chemistry
protein structure
biophysics
Macromolecular assemblies
microbiology
Virology
antivirals
Viral enzymes
Viral replication
Viral structure
Microbial pathogens
lasv
endonuclease
minigenomic
rna
transcription