Mutational analysis of LASV L endonuclease domain in the LASV minigenomic RNA transcription assay. Gregor D. Wallat Qinfeng Huang Wenjian Wang Haohao Dong Hinh Ly Yuying Liang Changjiang Dong 10.1371/journal.pone.0087577.g004 https://plos.figshare.com/articles/figure/_Mutational_analysis_of_LASV_L_endonuclease_domain_in_the_LASV_minigenomic_RNA_transcription_assay_/1080186 <p>FLAG-tagged L expression vector with either WT or alanine substitution at the respective residue within the N-terminal endonuclease domain was used to transfect 293T cells, together with the myc-tagged NP expression vector and LUC-encoded LASV MG RNA. LUC activity was measured and plotted in log scale. Results shown are the average of at least three independent experiments with error bars representing standard deviations. The expression of L (WT or mutant), NP, and GAPDH in the transfected cells was detected by Western blot analysis using anti-FLAG, anti-myc, and anti-GAPDH antibody, respectively.</p> 2014-02-07 11:36:37 Biochemistry proteins Protein chemistry protein structure biophysics Macromolecular assemblies microbiology Virology antivirals Viral enzymes Viral replication Viral structure Microbial pathogens lasv endonuclease minigenomic rna transcription