10.1371/journal.pone.0099993.g011 Irene Amigo-Jiménez Irene Amigo-Jiménez Elvira Bailón Elvira Bailón Estefanía Ugarte-Berzal Estefanía Ugarte-Berzal Noemí Aguilera-Montilla Noemí Aguilera-Montilla José A. García-Marco José A. García-Marco Angeles García-Pardo Angeles García-Pardo Culturing CLL cells on MMP-9 modulates Bcl-2 family proteins in response to ATO, preventing downregulation of Mcl-1, Bcl-xL and Bcl-2. Public Library of Science 2014 cell biology Cell processes Cell death Cellular stress responses Signal transduction cell signaling Signaling cascades Apoptotic signaling cascade Anti-apoptotic signaling Apoptotic signaling Molecular cell biology immunology Clinical immunology immunopathology hematology Hematologic cancers and related disorders leukemias Lymphoblastic leukemia Chronic lymphoblastic leukemia cll cells mmp-9 modulates bcl-2 proteins preventing downregulation bcl-xl 2014-06-23 02:54:57 Figure https://plos.figshare.com/articles/figure/_Culturing_CLL_cells_on_MMP_9_modulates_Bcl_2_family_proteins_in_response_to_ATO_preventing_downregulation_of_Mcl_1_Bcl_xL_and_Bcl_2_/1067490 <p>(A,B) 10×10<sup>6</sup> primary CLL cells in RPMI/0.1% FBS were incubated on BSA- or 150 nM MMP-9-coated wells for 1 h prior to adding 3 µM ATO or vehicle. After 24 h, cell were lysed and the indicated proteins (A) and ratios (B) analyzed by Western blotting as explained. * or <sup>#</sup>P≤0.05; ** or <sup>##</sup>P≤0.01; *** or <sup>###</sup>P≤0.001. Symbols are: *, CLL cells on BSA vs CLL cells on MMP-9; <sup>#</sup>, ATO-treated cells compared to their respective untreated controls.</p>