10.1371/journal.pone.0099993.g011
Irene Amigo-Jiménez
Irene
Amigo-Jiménez
Elvira Bailón
Elvira
Bailón
Estefanía Ugarte-Berzal
Estefanía
Ugarte-Berzal
Noemí Aguilera-Montilla
Noemí
Aguilera-Montilla
José A. García-Marco
José
A. García-Marco
Angeles García-Pardo
Angeles
García-Pardo
Culturing CLL cells on MMP-9 modulates Bcl-2 family proteins in response to ATO, preventing downregulation of Mcl-1, Bcl-xL and Bcl-2.
Public Library of Science
2014
cell biology
Cell processes
Cell death
Cellular stress responses
Signal transduction
cell signaling
Signaling cascades
Apoptotic signaling cascade
Anti-apoptotic signaling
Apoptotic signaling
Molecular cell biology
immunology
Clinical immunology
immunopathology
hematology
Hematologic cancers and related disorders
leukemias
Lymphoblastic leukemia
Chronic lymphoblastic leukemia
cll
cells
mmp-9
modulates
bcl-2
proteins
preventing
downregulation
bcl-xl
2014-06-23 02:54:57
Figure
https://plos.figshare.com/articles/figure/_Culturing_CLL_cells_on_MMP_9_modulates_Bcl_2_family_proteins_in_response_to_ATO_preventing_downregulation_of_Mcl_1_Bcl_xL_and_Bcl_2_/1067490
<p>(A,B) 10×10<sup>6</sup> primary CLL cells in RPMI/0.1% FBS were incubated on BSA- or 150 nM MMP-9-coated wells for 1 h prior to adding 3 µM ATO or vehicle. After 24 h, cell were lysed and the indicated proteins (A) and ratios (B) analyzed by Western blotting as explained. * or <sup>#</sup>P≤0.05; ** or <sup>##</sup>P≤0.01; *** or <sup>###</sup>P≤0.001. Symbols are: *, CLL cells on BSA vs CLL cells on MMP-9; <sup>#</sup>, ATO-treated cells compared to their respective untreated controls.</p>