AlsSD activity counters cytoplasmic acidification. ThomasVinai Chittezham SadykovMarat R. S. ChaudhariSujata JonesJoselyn L. EndresJennifer J. WidhelmTodd AhnJong-Sam S. JawaRandeep ZimmermanMatthew C. BaylesKenneth W. 2014 <p>(<b>A</b>) Over-expression of AlsS rescues pyruvate-mediated growth inhibition of Ī”<i>alsSD</i> mutant. (<b>B</b>) Intracellular pH (pHi) of UAMS-1 and derivatives following pyruvate stress. Bacterial cells loaded with the pH sensitive dye, cFSE, were suspended in potassium phosphate buffer (pH 4.5) and their pHi was measured as a function of the fluorescence emitted, either in the absence or presence of 40 mM sodium pyruvate (indicated by black arrow). (<b>C</b>) The rates of pHi recovery in each strain were measured from the slopes of the primary rise in pH<sub>i</sub> traces represented in panel B, (Statistical significance was assessed using one way ANOVA followed by Newman Kewl's multiple post-comparison test, nā€Š=ā€Š3; ** <i>P</i><0.005, *** <i>P</i><0.0005).</p>