10.1371/journal.ppat.1004205.g004
Vinai Chittezham Thomas
Vinai Chittezham
Thomas
Marat R. Sadykov
Marat R.
Sadykov
Sujata S. Chaudhari
Sujata
S. Chaudhari
Joselyn Jones
Joselyn
Jones
Jennifer L. Endres
Jennifer
L. Endres
Todd J. Widhelm
Todd
J. Widhelm
Jong-Sam Ahn
Jong-Sam
Ahn
Randeep S. Jawa
Randeep
S. Jawa
Matthew C. Zimmerman
Matthew C.
Zimmerman
Kenneth W. Bayles
Kenneth W.
Bayles
AlsSD activity counters cytoplasmic acidification.
Public Library of Science
2014
ecology
microbial ecology
biofilms
Bacterial biofilms
microbiology
bacteriology
Bacterial physiology
counters
cytoplasmic
2014-06-19 15:15:34
Figure
https://plos.figshare.com/articles/figure/_AlsSD_activity_counters_cytoplasmic_acidification_/1064203
<p>(<b>A</b>) Over-expression of AlsS rescues pyruvate-mediated growth inhibition of Ī<i>alsSD</i> mutant. (<b>B</b>) Intracellular pH (pHi) of UAMS-1 and derivatives following pyruvate stress. Bacterial cells loaded with the pH sensitive dye, cFSE, were suspended in potassium phosphate buffer (pH 4.5) and their pHi was measured as a function of the fluorescence emitted, either in the absence or presence of 40 mM sodium pyruvate (indicated by black arrow). (<b>C</b>) The rates of pHi recovery in each strain were measured from the slopes of the primary rise in pH<sub>i</sub> traces represented in panel B, (Statistical significance was assessed using one way ANOVA followed by Newman Kewl's multiple post-comparison test, nā=ā3; ** <i>P</i><0.005, *** <i>P</i><0.0005).</p>