10.1371/journal.ppat.1004205.g004 Vinai Chittezham Thomas Vinai Chittezham Thomas Marat R. Sadykov Marat R. Sadykov Sujata S. Chaudhari Sujata S. Chaudhari Joselyn Jones Joselyn Jones Jennifer L. Endres Jennifer L. Endres Todd J. Widhelm Todd J. Widhelm Jong-Sam Ahn Jong-Sam Ahn Randeep S. Jawa Randeep S. Jawa Matthew C. Zimmerman Matthew C. Zimmerman Kenneth W. Bayles Kenneth W. Bayles AlsSD activity counters cytoplasmic acidification. Public Library of Science 2014 ecology microbial ecology biofilms Bacterial biofilms microbiology bacteriology Bacterial physiology counters cytoplasmic 2014-06-19 15:15:34 Figure https://plos.figshare.com/articles/figure/_AlsSD_activity_counters_cytoplasmic_acidification_/1064203 <p>(<b>A</b>) Over-expression of AlsS rescues pyruvate-mediated growth inhibition of Ī”<i>alsSD</i> mutant. (<b>B</b>) Intracellular pH (pHi) of UAMS-1 and derivatives following pyruvate stress. Bacterial cells loaded with the pH sensitive dye, cFSE, were suspended in potassium phosphate buffer (pH 4.5) and their pHi was measured as a function of the fluorescence emitted, either in the absence or presence of 40 mM sodium pyruvate (indicated by black arrow). (<b>C</b>) The rates of pHi recovery in each strain were measured from the slopes of the primary rise in pH<sub>i</sub> traces represented in panel B, (Statistical significance was assessed using one way ANOVA followed by Newman Kewl's multiple post-comparison test, nā€Š=ā€Š3; ** <i>P</i><0.005, *** <i>P</i><0.0005).</p>