Validation of reference gene selection. YangQingpo LiZhen CaoJinjun ZhangSongdou ZhangHuaijiang WuXiaoyun ZhangQingwen LiuXiaoxia 2014 <p>(<b>A</b>) Relative Expression levels of <i>CHS1</i> in six developmental stages (<b>B</b>) Relative Expression levels of target gene, <i>GSTs1</i>, in midgut (MG), Malpighian tubules (MT), fatbodies (FB), Spermary (SP) (<b>C</b>) and different insecticides, cyhalothrin (EC1), acetamiprid (EC2), chlorantraniliprole (EC3), chlorpyrifos (EC4). NF1, using the single one best reference gene for normalization; NF (1–2), using two best reference genes; NF (1–3), using three best reference genes; NF (1–5) using four best reference genes; NF (12), using the least stable reference gene. Asterisks indicate significant difference of the expression of the target gene based on three biological replications. (<i>P</i><0.05, t-test; n = 3).</p>