10.1371/journal.pone.0098164.g005 Qingpo Yang Qingpo Yang Zhen Li Zhen Li Jinjun Cao Jinjun Cao Songdou Zhang Songdou Zhang Huaijiang Zhang Huaijiang Zhang Xiaoyun Wu Xiaoyun Wu Qingwen Zhang Qingwen Zhang Xiaoxia Liu Xiaoxia Liu Validation of reference gene selection. Public Library of Science 2014 cell biology Molecular cell biology genetics gene expression Gene identification and analysis Molecular genetics 2014-06-02 04:43:13 Figure https://plos.figshare.com/articles/figure/_Validation_of_reference_gene_selection_/1042668 <p>(<b>A</b>) Relative Expression levels of <i>CHS1</i> in six developmental stages (<b>B</b>) Relative Expression levels of target gene, <i>GSTs1</i>, in midgut (MG), Malpighian tubules (MT), fatbodies (FB), Spermary (SP) (<b>C</b>) and different insecticides, cyhalothrin (EC1), acetamiprid (EC2), chlorantraniliprole (EC3), chlorpyrifos (EC4). NF1, using the single one best reference gene for normalization; NF (1–2), using two best reference genes; NF (1–3), using three best reference genes; NF (1–5) using four best reference genes; NF (12), using the least stable reference gene. Asterisks indicate significant difference of the expression of the target gene based on three biological replications. (<i>P</i><0.05, t-test; n = 3).</p>