10.1371/journal.pone.0098164.g005
Qingpo Yang
Qingpo
Yang
Zhen Li
Zhen
Li
Jinjun Cao
Jinjun
Cao
Songdou Zhang
Songdou
Zhang
Huaijiang Zhang
Huaijiang
Zhang
Xiaoyun Wu
Xiaoyun
Wu
Qingwen Zhang
Qingwen
Zhang
Xiaoxia Liu
Xiaoxia
Liu
Validation of reference gene selection.
Public Library of Science
2014
cell biology
Molecular cell biology
genetics
gene expression
Gene identification and analysis
Molecular genetics
2014-06-02 04:43:13
Figure
https://plos.figshare.com/articles/figure/_Validation_of_reference_gene_selection_/1042668
<p>(<b>A</b>) Relative Expression levels of <i>CHS1</i> in six developmental stages (<b>B</b>) Relative Expression levels of target gene, <i>GSTs1</i>, in midgut (MG), Malpighian tubules (MT), fatbodies (FB), Spermary (SP) (<b>C</b>) and different insecticides, cyhalothrin (EC1), acetamiprid (EC2), chlorantraniliprole (EC3), chlorpyrifos (EC4). NF1, using the single one best reference gene for normalization; NF (1–2), using two best reference genes; NF (1–3), using three best reference genes; NF (1–5) using four best reference genes; NF (12), using the least stable reference gene. Asterisks indicate significant difference of the expression of the target gene based on three biological replications. (<i>P</i><0.05, t-test; n = 3).</p>