10.1371/journal.pone.0097359
Chunfan Jiang
Chunfan
Jiang
Tao Huang
Tao
Huang
Yun Wang
Yun
Wang
Guowei Huang
Guowei
Huang
Xia Wan
Xia
Wan
Jiang Gu
Jiang
Gu
Immunoglobulin G Expression in Lung Cancer and Its Effects on Metastasis
Public Library of Science
2014
Biochemistry
proteins
Immune system proteins
antibodies
cell biology
Cell processes
Cell growth
Molecular cell biology
genetics
gene expression
immunology
Clinical immunology
immunopathology
oncology
Basic cancer research
metastasis
Cancers and neoplasms
carcinomas
adenocarcinomas
Adenocarcinoma of the lung
Squamous cell carcinomas
Squamous cell lung carcinoma
Lung and intrathoracic tumors
Cancer detection and diagnosis
Pathology and laboratory medicine
Clinical pathology
cancer
2014-05-22 03:31:30
Dataset
https://plos.figshare.com/articles/dataset/_Immunoglobulin_G_Expression_in_Lung_Cancer_and_Its_Effects_on_Metastasis_/1034694
<div><p>Lung cancer is one of the leading malignancies worldwide, but the regulatory mechanism of its growth and metastasis is still poorly understood. We investigated the possible expression of immunoglobulin G (IgG) genes in squamous cell carcinomas and adenocarcinomas of the lung and related cancer cell lines. Abundant mRNA of IgG and essential enzymes for IgG synthesis, recombination activation genes 1, 2 (RAG1, 2) and activation-induced cytidine deaminase (AID) were detected in the cancer cells but not in adjacent normal lung tissue or normal lung epithelial cell line. The extents of IgG expression in 86 lung cancers were found to associate with clinical stage, pathological grade and lymph node metastasis. We found that knockdown of IgG with siRNA resulted in decreases of cellular proliferation, migration and attachment for cultured lung cancer cells. Metastasis-associated gene 1 (MTA1) appeared to be co-expressed with IgG in lung cancer cells. Statistical analysis showed that the rate of IgG expression was significantly correlated to that of MTA1 and to lymph node metastases. Inhibition of MTA1 gene expression with siRNA also led to decreases of cellular migration and attachment for cultured lung cancer cells. These evidences suggested that inhibition of cancer migration and attachment induced by IgG down-regulation might be achieved through MTA1 regulatory pathway. Our findings suggest that lung cancer-produced IgG is likely to play an important role in cancer growth and metastasis with significant clinical implications.</p></div>