10.1371/journal.ppat.1008014.g009
Jennifer R. Linden
Jennifer R.
Linden
Claudia Flores
Claudia
Flores
Eric F. Schmidt
Eric F.
Schmidt
Francisco A. Uzal
Francisco A.
Uzal
Adam O. Michel
Adam O.
Michel
Marissa Valenzuela
Marissa
Valenzuela
Sebastian Dobrow
Sebastian
Dobrow
Timothy Vartanian
Timothy
Vartanian
ETX treatment causes caveolae formation.
Public Library of Science
2019
RAB
MAL Clostridium perfringens epsilon toxin
Clostridium perfringens epsilon toxin
endosome
EEA
Internalized caveolae fuse
lipid raft-dependent vacuolation
60 kDA serum albumin
155 kDA IgG
multivesicular bodies fuse basally
CNS microvasculature
caveolae-dependent transcytosis
ETX causes blood brain barrier
70 kDa dextran
ETX causes BBB permeability
376 Da fluorescein salt
ETX binding
multivesicular bodies
blood brain barrier permeability
ETX-induced BBB permeability
exhibit ETX-induced BBB permeability
2019-11-08 18:34:45
Figure
https://plos.figshare.com/articles/figure/ETX_treatment_causes_caveolae_formation_/10277540
<p>(A) SEM of primary BEC treated with or without 50nM of ETX for 1 hour. White arrows point to apical surface invaginations. (B) Higher magnification of surface invaginations on ETX treated BEC. (C) Vertical TEM sections of primary BEC treated with or without 50nM ETX for 2 hours. White arrows point to apical surface invaginations morphologically similar to caveolae. (D) Quantification of the number of caveolae per um of cell surface in control or ETX treated cells. Results expressed as Mean ± STDEV, *p<0.003 determined by T-Test, n = 3. (E) and (F) additional micrographs of BEC treated with 50nM ETX for 2 hours. Black arrows denote caveolae fusing into other internalized caveolae or endosomes. In some cells, large MVB are observed.</p>