%0 Figure %A Linden, Jennifer R. %A Flores, Claudia %A Schmidt, Eric F. %A Uzal, Francisco A. %A Michel, Adam O. %A Valenzuela, Marissa %A Dobrow, Sebastian %A Vartanian, Timothy %D 2019 %T High magnification of Mal+/+ and Mal-/- intravenously injected with ETX-594. %U https://plos.figshare.com/articles/figure/High_magnification_of_i_Mal_i_and_i_Mal-_-_i_intravenously_injected_with_ETX-594_/10277525 %R 10.1371/journal.ppat.1008014.g004 %2 https://plos.figshare.com/ndownloader/files/18608066 %K RAB %K MAL Clostridium perfringens epsilon toxin %K Clostridium perfringens epsilon toxin %K endosome %K EEA %K Internalized caveolae fuse %K lipid raft-dependent vacuolation %K 60 kDA serum albumin %K 155 kDA IgG %K multivesicular bodies fuse basally %K CNS microvasculature %K caveolae-dependent transcytosis %K ETX causes blood brain barrier %K 70 kDa dextran %K ETX causes BBB permeability %K 376 Da fluorescein salt %K ETX binding %K multivesicular bodies %K blood brain barrier permeability %K ETX-induced BBB permeability %K exhibit ETX-induced BBB permeability %X

Wild type mice expressing Mal+/+ or mice deficient in Mal-/- were intravenously injected with Alexa Fluor 594 conjugated ETX (ETX-594) for ten minutes then perfused with PBS to remove unbound toxin. ETX binding to kidney tissue was evaluated by fluorescent microscopy. White arrows indicate ETX-594 binding to renal tubules. White asterisks indicate accumulation of ETX-594 in unknown renal compartment.

%I PLOS Pathogens