%0 Figure %A Takeuchi, Hiroki %A Sasaki, Naoko %A Yamaga, Shunsuke %A Kuboniwa, Masae %A Matsusaki, Michiya %A Amano, Atsuo %D 2019 %T P. gingivalis gingipains degrade JAM1 in IHGE cells. %U https://plos.figshare.com/articles/figure/_i_P_i_i_gingivalis_i_gingipains_degrade_JAM1_in_IHGE_cells_/10269326 %R 10.1371/journal.ppat.1008124.g001 %2 https://plos.figshare.com/ndownloader/files/18547616 %K Lys-specific cysteine proteases %K Gingival epithelial cells %K LPS %K degradation %K JAM 1 homodimers %K 40 kDa dextran %K gingipain %K gingival epithelial interface %K gingival epithelial cells %K JAM 1. Knockdown %K JAM 1 %K PGN %K junctional adhesion molecule %K junctional adhesion molecule 1 Porphyromonas gingivalis %K gingival epithelium %K epithelial barrier function %X

(A) IHGE cells were infected for 1 h with P. gingivalis WT or the Δkgp ΔrgpA ΔrgpB mutant at an MOI of 100. The cells were then analyzed by immunoblotting with the indicated antibodies. (B–E) IHGE cells were transiently transfected with plasmid encoding Myc-mCherry-CLDN1 (B), Myc-mCherry-CLDN4 (C), Myc-mCherry-OCLN (D), or HA-TJP1 (E). After 48 h of incubation, cells were infected with P. gingivalis at an MOI of 100 for 1 or 3 h. The cells were then analyzed by immunoblotting with the indicated antibodies. β-actin was used as a loading control. IB, immunoblot.

%I PLOS Pathogens