<i>P</i>. <i>gingivalis</i> gingipains degrade JAM1 in IHGE cells. Hiroki Takeuchi Naoko Sasaki Shunsuke Yamaga Masae Kuboniwa Michiya Matsusaki Atsuo Amano 10.1371/journal.ppat.1008124.g001 https://plos.figshare.com/articles/figure/_i_P_i_i_gingivalis_i_gingipains_degrade_JAM1_in_IHGE_cells_/10269326 <p><b>(A)</b> IHGE cells were infected for 1 h with <i>P</i>. <i>gingivalis</i> WT or the Δ<i>kgp</i> Δ<i>rgpA</i> Δ<i>rgpB</i> mutant at an MOI of 100. The cells were then analyzed by immunoblotting with the indicated antibodies. <b>(B–E)</b> IHGE cells were transiently transfected with plasmid encoding Myc-mCherry-CLDN1 (B), Myc-mCherry-CLDN4 (C), Myc-mCherry-OCLN (D), or HA-TJP1 (E). After 48 h of incubation, cells were infected with <i>P</i>. <i>gingivalis</i> at an MOI of 100 for 1 or 3 h. The cells were then analyzed by immunoblotting with the indicated antibodies. β-actin was used as a loading control. IB, immunoblot.</p> 2019-11-07 18:59:48 Lys-specific cysteine proteases Gingival epithelial cells LPS degradation JAM 1 homodimers 40 kDa dextran gingipain gingival epithelial interface gingival epithelial cells JAM 1. Knockdown JAM 1 PGN junctional adhesion molecule junctional adhesion molecule 1 Porphyromonas gingivalis gingival epithelium epithelial barrier function