10.1371/journal.ppat.1008124.g001
Hiroki Takeuchi
Hiroki
Takeuchi
Naoko Sasaki
Naoko
Sasaki
Shunsuke Yamaga
Shunsuke
Yamaga
Masae Kuboniwa
Masae
Kuboniwa
Michiya Matsusaki
Michiya
Matsusaki
Atsuo Amano
Atsuo
Amano
<i>P</i>. <i>gingivalis</i> gingipains degrade JAM1 in IHGE cells.
Public Library of Science
2019
Lys-specific cysteine proteases
Gingival epithelial cells
LPS
degradation
JAM 1 homodimers
40 kDa dextran
gingipain
gingival epithelial interface
gingival epithelial cells
JAM 1. Knockdown
JAM 1
PGN
junctional adhesion molecule
junctional adhesion molecule 1 Porphyromonas gingivalis
gingival epithelium
epithelial barrier function
2019-11-07 18:59:48
Figure
https://plos.figshare.com/articles/figure/_i_P_i_i_gingivalis_i_gingipains_degrade_JAM1_in_IHGE_cells_/10269326
<p><b>(A)</b> IHGE cells were infected for 1 h with <i>P</i>. <i>gingivalis</i> WT or the Δ<i>kgp</i> Δ<i>rgpA</i> Δ<i>rgpB</i> mutant at an MOI of 100. The cells were then analyzed by immunoblotting with the indicated antibodies. <b>(B–E)</b> IHGE cells were transiently transfected with plasmid encoding Myc-mCherry-CLDN1 (B), Myc-mCherry-CLDN4 (C), Myc-mCherry-OCLN (D), or HA-TJP1 (E). After 48 h of incubation, cells were infected with <i>P</i>. <i>gingivalis</i> at an MOI of 100 for 1 or 3 h. The cells were then analyzed by immunoblotting with the indicated antibodies. β-actin was used as a loading control. IB, immunoblot.</p>