10.1371/journal.pgen.1004302.g008 Sandra Tenreiro Sandra Tenreiro Madalena M. Reimão-Pinto Madalena M. Reimão-Pinto Pedro Antas Pedro Antas José Rino José Rino Donata Wawrzycka Donata Wawrzycka Diana Macedo Diana Macedo Rita Rosado-Ramos Rita Rosado-Ramos Triana Amen Triana Amen Meytal Waiss Meytal Waiss Filipa Magalhães Filipa Magalhães Andreia Gomes Andreia Gomes Cláudia N. Santos Cláudia N. Santos Daniel Kaganovich Daniel Kaganovich Tiago Fleming Outeiro Tiago Fleming Outeiro Inclusions formed by S129A aSyn-GFP are more heterogeneous than the ones formed by WT aSyn-GFP. Public Library of Science 2014 neuroscience Cellular neuroscience Molecular neuroscience Model organisms Yeast and fungal models formed s129a asyn-gfp heterogeneous ones wt 2014-05-08 02:51:33 Figure https://plos.figshare.com/articles/figure/_Inclusions_formed_by_S129A_aSyn_GFP_are_more_heterogeneous_than_the_ones_formed_by_WT_aSyn_GFP_/1020125 <p>(A) FRAP recovery curves for WT and S129A aSyn-GFP inclusions on yeast cells expressing aSyn forms at the indicated time points. For each strain, at least 15 inclusions in different cells were analyzed. Each plot represents mean ± SD for each time point for all FRAP experiments (for the WT aSyn-GFP expressing cells) or groups of FRAP experiments organized by recovery profiles (for the S129A aSyn-GFP expressing cells). (B) FRAP time lapse recording of a representative inclusion of each group (C) Area (pixels) and fluorescence intensity mean (in arbitrary units, a.u.) of the inclusions analyzed (***p<0.001 and ** p<0.01; one way ANOVA with Bonferroni's multiple comparison test). A representative result is shown from at least three independent experiments. Values represent the mean ± SD.</p>