10.1371/journal.pone.0096250.g001
Linda Ravenna
Linda
Ravenna
Lorenzo Principessa
Lorenzo
Principessa
Alessandra Verdina
Alessandra
Verdina
Luisa Salvatori
Luisa
Salvatori
Matteo Antonio Russo
Matteo Antonio
Russo
Elisa Petrangeli
Elisa
Petrangeli
Time-course of hypoxia on HIF1α, HIF2α and HIF3α protein nuclear level and mRNA expression in LNCaP, DU145 and PC3 cells.
Public Library of Science
2014
Biochemistry
Oxidative damage
cell biology
Cell processes
Cellular stress responses
Molecular cell biology
genetics
gene expression
DNA transcription
Protein translation
immunology
Immune response
inflammation
Clinical immunology
immunity
oncology
Cancers and neoplasms
Genitourinary tract tumors
Prostate cancer
Basic cancer research
hypoxia
mrna
du145
pc3
2014-05-06 02:56:37
Figure
https://plos.figshare.com/articles/figure/_Time_course_of_hypoxia_on_HIF1_945_HIF2_945_and_HIF3_945_protein_nuclear_level_and_mRNA_expression_in_LNCaP_DU145_and_PC3_cells_/1017636
<p>Cell were exposed to 1% O<sub>2</sub> from 1 up to 48–72 h, according to the experimental design or left untreated. <b>A</b> Protein expression was detected in nuclear extracts by immunoblot analysis. A representative experiment for each gene in each studied cell lines is shown. β-actin was used as a loading control. <b>B</b> mRNA determinations were performed by real-time PCR, normalized to the housekeeping gene 18S rRNA and expressed as fold induction with respect to normoxic controls (set at 1). Mean values ± SE.</p>