10.1371/journal.pone.0096250.g001 Linda Ravenna Linda Ravenna Lorenzo Principessa Lorenzo Principessa Alessandra Verdina Alessandra Verdina Luisa Salvatori Luisa Salvatori Matteo Antonio Russo Matteo Antonio Russo Elisa Petrangeli Elisa Petrangeli Time-course of hypoxia on HIF1α, HIF2α and HIF3α protein nuclear level and mRNA expression in LNCaP, DU145 and PC3 cells. Public Library of Science 2014 Biochemistry Oxidative damage cell biology Cell processes Cellular stress responses Molecular cell biology genetics gene expression DNA transcription Protein translation immunology Immune response inflammation Clinical immunology immunity oncology Cancers and neoplasms Genitourinary tract tumors Prostate cancer Basic cancer research hypoxia mrna du145 pc3 2014-05-06 02:56:37 Figure https://plos.figshare.com/articles/figure/_Time_course_of_hypoxia_on_HIF1_945_HIF2_945_and_HIF3_945_protein_nuclear_level_and_mRNA_expression_in_LNCaP_DU145_and_PC3_cells_/1017636 <p>Cell were exposed to 1% O<sub>2</sub> from 1 up to 48–72 h, according to the experimental design or left untreated. <b>A</b> Protein expression was detected in nuclear extracts by immunoblot analysis. A representative experiment for each gene in each studied cell lines is shown. β-actin was used as a loading control. <b>B</b> mRNA determinations were performed by real-time PCR, normalized to the housekeeping gene 18S rRNA and expressed as fold induction with respect to normoxic controls (set at 1). Mean values ± SE.</p>