10.1371/journal.pone.0096253.g001
Magali Saint-Geniez
Magali
Saint-Geniez
Elisa Ghelfi
Elisa
Ghelfi
Xiaoliang Liang
Xiaoliang
Liang
Chenwei Yu
Chenwei
Yu
Carrie Spencer
Carrie
Spencer
Stephanie Abend
Stephanie
Abend
Gokhan Hotamisligil
Gokhan
Hotamisligil
Sule Cataltepe
Sule
Cataltepe
FABP4 expression and function during normal retinal development.
Public Library of Science
2014
anatomy
biological tissue
epithelium
Epithelial cells
Endothelial cells
Cardiovascular anatomy
Biochemistry
lipids
cell biology
Cellular types
Molecular cell biology
developmental biology
Organism development
genetics
gene expression
cardiology
ophthalmology
Retinal disorders
pediatrics
Child development
retinal
2014-05-06 02:57:11
Figure
https://plos.figshare.com/articles/figure/_FABP4_expression_and_function_during_normal_retinal_development_/1017623
<p>(A) qPCR quantification of FABP4 mRNA in total retinas from P1 to P33 WT mice. Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3–4). FABP4 is significantly induced during the postnatal stage of retinal development. (B) FABP4 distribution was determined on retina flat-mounts and paraffin sections. At P7, FABP4 expression (red) was not observed in the inner retinal vessels stained by the pan-EC marker EMCN (green). FABP4 was strongly detected in the vitreal macrophages (arrowheads) and hyaloid endothelial cells (arrows). Paraffin sections of adult retina demonstrate faint, but uniform expression of FABP4 in all retinal layers. (C) WT and FABP4<sup>−/−</sup> P7 flat-mount retinas were immunostained with Collagen IV to identify the vascular network. (D) Vascular coverage and density was similar in WT and FABP4<sup>−/−</sup> animals indicating that FABP4 expression is not required for normal retinal vascularization. (E) Hematoxylin and eosin stained sagittal retinal sections of adult WT and FABP4<sup>−/−</sup> mice showed no morphological anomalies or degeneration in FABP4<sup>−/−</sup> animals. gcl: ganglion cell layer; ipl: inner plexiform layer; inl: inner nuclear layer; onl: outer nuclear layer; os: outer segment.</p>