Figure S1 - Recombinant Expression and Characterization of the Cytoplasmic Rice β-Glucosidase Os1BGlu4 Chen Rouyi Supaporn Baiya Sang-Kyu Lee Bancha Mahong Jong-Seong Jeon James R. Ketudat-Cairns Mariena Ketudat-Cairns 10.1371/journal.pone.0096712.s001 https://plos.figshare.com/articles/figure/_Recombinant_Expression_and_Characterization_of_the_Cytoplasmic_Rice_946_Glucosidase_Os1BGlu4_/1017621 <p><b>Subcellular localization of Os1BGlu4-GFP (A-D) and GFP-Os1BGlu4 (E-H) fusion proteins in tobacco epidermal cells and western blot analysis of the Os1BGlu4-GFP and GFP-Os1BGlu4 fusion proteins.</b> Fluorescent GFP signals (A, E), propidium iodide stained nuclei (B, F) chlorophyll autofluorescence (C, G) and merged images (D, H) are shown. (I) Western blot analysis of Os1BGlu4-GFP and GFP-Os1BGlu4 fusion proteins extracted from the leaf cells with an anti-GFP antibody, which shows that the protein is intact and no cleaved GFP is detectable. C, chloroplast; N, nucleus; V, vacuole. The bar in the merged images, 10 µm.</p> <p>(TIF)</p> 2014-05-06 02:54:02 Biochemistry biosynthesis Carbohydrate biosynthesis enzymology Enzyme kinetics enzymes proteins protein synthesis Recombinant proteins biocatalysis Plant biochemistry genetics gene expression DNA transcription Protein translation genomics Functional genomics Gene function characterization cytoplasmic