%0 Figure %A Glaser, Talita %A La Banca de Oliveira, Sophia %A Cheffer, Arquimedes %A Beco, Renata %A Martins, Patrícia %A Fornazari, Maynara %A Lameu, Claudiana %A Miranda Costa Junior, Helio %A Coutinho-Silva, Robson %A Ulrich, Henning %D 2014 %T Modulation of neuroectodermal differentiation of mouse ESC by P2X7R activity. %U https://plos.figshare.com/articles/figure/_Modulation_of_neuroectodermal_differentiation_of_mouse_ESC_by_P2X7R_activity_/1016209 %R 10.1371/journal.pone.0096281.g008 %2 https://plos.figshare.com/ndownloader/files/1484820 %K cell biology %K Cell processes %K Cell growth %K Cellular types %K Animal cells %K stem cells %K Embryonic stem cells %K Signal transduction %K cell signaling %K Developmental signaling %K Molecular cell biology %K developmental biology %K Cell differentiation %K Cell fate determination %K Molecular development %K genetics %K gene expression %K neuroscience %K Developmental neuroscience %K neuroectodermal %K differentiation %K esc %K p2x7r %X

(A) Flow cytometry analysis of SSEA-1 and β3-tubulin protein expression in neural-differentiated ES cells. Cells were cultured and induced to differentiation as described in Materials and Methods. (B) Immunofluorescence assay of SSEA-1 and β3-tubulin expression of cells following 8 days of differentiation. Circled areas: 1. cells expressing only SSEA-1, 2. cells co-expressing SSEA-1 and β3-tubulin. Scale bar, 50 µm. (C) Flow cytometry analysis of SSEA-1-positive cells co-expressing or not β3-tubulin on day 8 of differentiation cultured in the presence of 10 µM Bz-ATP or 10 µM KN-62, respectively. (D) Percentage of β3-tubulin-positive cells differentiated in the absence or presence of 1 µM A438079 or 10 µM KN-62, as determined by flow cytometry. Statistical relevance was analyzed by the One-Way ANOVA test followed by the Bonferroni post-hoc test. Six independent experiments were performed (* p<0.05 compared to control data).

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