Glaser, Talita La Banca de Oliveira, Sophia Cheffer, Arquimedes Beco, Renata Martins, Patrícia Fornazari, Maynara Lameu, Claudiana Miranda Costa Junior, Helio Coutinho-Silva, Robson Ulrich, Henning Proliferation of ESC in conditions of P2X7R modulation. <p>Cell cycle analysis based on flow cytometric analysis of BrdU incorporation and propidium iodide DNA-staining were performed as described in Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096281#s2" target="_blank">Methods</a>. (<b>A</b>) Cell distributions at different S-phases of ESC treated with P2X7R agonist or inhibitor, 0.1 µM and 1 µM Bz-ATP and 10 µM KN-62 for 96 h, respectively. Shown data are representative for mean values ±S.E. of five independent experiments. Data were statistical analyzed by the One-Way ANOVA test followed by the Bonferroni post hoc test with (*p<0,05 compared to control data). (<b>B</b>) Representative BrdU/PI cell cycle analysis of ESC treated with Bz-ATP or KN-62 compared to untreated control cultures. (<b>C</b>). Cell cycle distributions of ESC treated for 96 h with the P2X7R agonist (0.1 µM or 1 µM Bz-ATP) or the P2X7R inhibitor KN-62 (10 µM), respectively. Shown data are representative for mean values ±S.E. of five independent experiments.</p> cell biology;Cell processes;Cell growth;Cellular types;Animal cells;stem cells;Embryonic stem cells;Signal transduction;cell signaling;Developmental signaling;Molecular cell biology;developmental biology;Cell differentiation;Cell fate determination;Molecular development;genetics;gene expression;neuroscience;Developmental neuroscience;esc;p2x7r 2014-05-05
    https://plos.figshare.com/articles/figure/_Proliferation_of_ESC_in_conditions_of_P2X7R_modulation_/1016203
10.1371/journal.pone.0096281.g005