Proliferation of ESC in conditions of P2X7R modulation.
Talita Glaser
Sophia La Banca de Oliveira
Arquimedes Cheffer
Renata Beco
Patrícia Martins
Maynara Fornazari
Claudiana Lameu
Helio Miranda Costa Junior
Robson Coutinho-Silva
Henning Ulrich
10.1371/journal.pone.0096281.g005
https://plos.figshare.com/articles/figure/_Proliferation_of_ESC_in_conditions_of_P2X7R_modulation_/1016203
<p>Cell cycle analysis based on flow cytometric analysis of BrdU incorporation and propidium iodide DNA-staining were performed as described in Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096281#s2" target="_blank">Methods</a>. (<b>A</b>) Cell distributions at different S-phases of ESC treated with P2X7R agonist or inhibitor, 0.1 µM and 1 µM Bz-ATP and 10 µM KN-62 for 96 h, respectively. Shown data are representative for mean values ±S.E. of five independent experiments. Data were statistical analyzed by the One-Way ANOVA test followed by the Bonferroni post hoc test with (*p<0,05 compared to control data). (<b>B</b>) Representative BrdU/PI cell cycle analysis of ESC treated with Bz-ATP or KN-62 compared to untreated control cultures. (<b>C</b>). Cell cycle distributions of ESC treated for 96 h with the P2X7R agonist (0.1 µM or 1 µM Bz-ATP) or the P2X7R inhibitor KN-62 (10 µM), respectively. Shown data are representative for mean values ±S.E. of five independent experiments.</p>
2014-05-05 03:29:37
cell biology
Cell processes
Cell growth
Cellular types
Animal cells
stem cells
Embryonic stem cells
Signal transduction
cell signaling
Developmental signaling
Molecular cell biology
developmental biology
Cell differentiation
Cell fate determination
Molecular development
genetics
gene expression
neuroscience
Developmental neuroscience
esc
p2x7r