Proliferation of ESC in conditions of P2X7R modulation. Talita Glaser Sophia La Banca de Oliveira Arquimedes Cheffer Renata Beco Patrícia Martins Maynara Fornazari Claudiana Lameu Helio Miranda Costa Junior Robson Coutinho-Silva Henning Ulrich 10.1371/journal.pone.0096281.g005 https://plos.figshare.com/articles/figure/_Proliferation_of_ESC_in_conditions_of_P2X7R_modulation_/1016203 <p>Cell cycle analysis based on flow cytometric analysis of BrdU incorporation and propidium iodide DNA-staining were performed as described in Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096281#s2" target="_blank">Methods</a>. (<b>A</b>) Cell distributions at different S-phases of ESC treated with P2X7R agonist or inhibitor, 0.1 µM and 1 µM Bz-ATP and 10 µM KN-62 for 96 h, respectively. Shown data are representative for mean values ±S.E. of five independent experiments. Data were statistical analyzed by the One-Way ANOVA test followed by the Bonferroni post hoc test with (*p<0,05 compared to control data). (<b>B</b>) Representative BrdU/PI cell cycle analysis of ESC treated with Bz-ATP or KN-62 compared to untreated control cultures. (<b>C</b>). Cell cycle distributions of ESC treated for 96 h with the P2X7R agonist (0.1 µM or 1 µM Bz-ATP) or the P2X7R inhibitor KN-62 (10 µM), respectively. Shown data are representative for mean values ±S.E. of five independent experiments.</p> 2014-05-05 03:29:37 cell biology Cell processes Cell growth Cellular types Animal cells stem cells Embryonic stem cells Signal transduction cell signaling Developmental signaling Molecular cell biology developmental biology Cell differentiation Cell fate determination Molecular development genetics gene expression neuroscience Developmental neuroscience esc p2x7r