10.1371/journal.pone.0224221.g008
Tapan Goel
Tapan
Goel
Rui Wang
Rui
Wang
Sara Martin
Sara
Martin
Elizabeth Lanphear
Elizabeth
Lanphear
Eva-Maria S. Collins
Eva-Maria S.
Collins
<i>Hydra</i> response to 1mM linalool (L), 0.04% heptanol (H), 2% urethane (U), and 0.1% chloretone (C).
Public Library of Science
2019
anesthetic
Hydra studies
vivo imaging
transplantation experiments
vivo studies
monoterpenoid alcohol linalool
light stimuli
cell proliferation
tissue manipulation
head regeneration
Hydra research
Hydra regeneration
teaching contexts
animal health
Hydra lines
cell viability
Linalool acts
2019-10-24 17:47:25
Figure
https://plos.figshare.com/articles/figure/_i_Hydra_i_response_to_1mM_linalool_L_0_04_heptanol_H_2_urethane_U_and_0_1_chloretone_C_/10045046
<p>A. Percent length of anesthetized <i>Hydra</i> polyps compared to their natural state at 10min incubation. n = 10 animals per condition across 2 technical replicates. Anesthetized lengths similar to the average lengths in HM were recorded in linalool at 103% (87, 112; median (25<sup>th</sup> percentile, 75<sup>th</sup> percentile)), heptanol at 83% (71, 93) and urethane at 96% (88, 118), while chloretone-treated animals hyperextended at 133% (125, 153). B. Induction times across 2 technical replicates. Linalool n = 13, heptanol n = 14, urethane n = 13, chloretone n = 10. Linalool’s median induction time was 9 min (6, 9) (median (25<sup>th</sup> percentile, 75<sup>th</sup> percentile)) and thus significantly longer than that of heptanol at 6 min (4, 9), urethane at 5 min (4, 6) and chloretone at 5 min (3, 7). C. Recovery times across 2 technical replicates. Linalool n = 10, heptanol n = 8, urethane n = 12, chloretone n = 10. Median recovery time was 8 min (7, 17) for linalool, 11 min (7,15) for heptanol, 14 min (12, 26) for urethane and 13 min (7,15) for chloretone. (D-F) Pairwise statistical comparisons of data shown in A-C. Pink, red and dark red indicate a statistically significant difference at p<0.05, p<0.01 and p<0.001 respectively, determined using the Mann-Whitney U test between pairs of anesthetics. D. Comparison between percent length distributions. E. Comparison between induction time distributions. F. Comparison between recovery time distributions. G. Overview of the four anesthetics tested, scored on degree of immobilization, animal health following anesthesia, time to induce anesthesia, time to recover from anesthesia, morphology, and ease of use (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0224221#sec010" target="_blank">Methods</a>).</p>