%0 Figure %A P. Abreu, Thiago %A S. Silva, Leandro %A M. Takiya, Christina %A C. Souza, Mariana %A G. Henriques, Maria %A Acacia S. Pinheiro, Ana %A Caruso-Neves, Celso %D 2014 %T Experimental design. %U https://plos.figshare.com/articles/figure/_Experimental_design_/1000575 %R 10.1371/journal.pone.0093634.g001 %2 https://plos.figshare.com/ndownloader/files/1465983 %K anatomy %K Renal system %K microbiology %K physiology %K Renal physiology %K Pathology and laboratory medicine %K pathogenesis %K Host-pathogen interactions %X

(A) C57BL/6 mice were separated into four experimental groups that were euthanized on day 5 (5d) or day 21 (21d) post infection (p.i.): noninfected group (control5d); mice infected with 106 infected red blood cells euthanized on day 5 p.i. (infected5d); noninfected mice that received treatment with antimalarials (control+treated21d); and the Plasmodium berghei ANKA (PbA)-infected group treated with antimalarials and euthanized on day 21 p.i. (infected+treated21d). (B) Additional experimental groups that were euthanized on day 21 underwent or not a second kidney insult induced with intraperitoneal injections of bovine serum albumin (BSA): noninfected group (control21d); noninfected mice with BSA-induced renal injury (control+BSA21d); noninfected mice that received treatment with antimalarials and subsequent BSA injections (control+treated+BSA21d); PbA-infected mice treated with antimalarials (infected+treated21d); and PbA-infected mice that were treated and subsequently underwent a second kidney insult (infected+treated+BSA21d). (C) Parasitemia was determined from blood smears stained with Diff-Quick in all infected groups on day 1 (A), day 5 (B), day 8 (C), and day 21 (D) p.i.

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