ATHB17Δ113 forms homodimers and makes heterodimers with maize HD-Zip II proteins. Elena A. Rice Abha Khandelwal Robert A. Creelman Cara Griffith Jeffrey E. Ahrens J. Philip Taylor Lesley R. Murphy Siva Manjunath Rebecca L. Thompson Matthew J. Lingard Stephanie L. Back Huachun Larue Bonnie R. Brayton Amanda J. Burek Shiv Tiwari Luc Adam James A. Morrell Rico A. Caldo Qing Huai Jean-Louis K. Kouadio Rosemarie Kuehn Anagha M. Sant William J. Wingbermuehle Rodrigo Sala Matt Foster Josh D. Kinser Radha Mohanty Dongming Jiang Todd E. Ziegler Mingya G. Huang Saritha V. Kuriakose Kyle Skottke Peter P. Repetti T. Lynne Reuber Thomas G. Ruff Marie E. Petracek Paul J. Loida 10.1371/journal.pone.0094238.g006 https://plos.figshare.com/articles/figure/_ATHB17_916_113_forms_homodimers_and_makes_heterodimers_with_maize_HD_Zip_II_proteins_/1000552 <p>Maize leaf protoplasts were (A) mock- transformed or transformed with constructs expressing CFP, MYC::HA and CFP tagged ATHB17Δ113 alone or CFP and ATHB17Δ113::CFP in combination with ATHB17Δ113::MYC construct, (B) transformed (filled bars) with constructs expressing CFP or each of the CFP tagged HD-Zip IIs alone or co-transformed (empty bars) with the construct expressing ATHB17Δ113::MYC-HA. Cellular extracts were co-immunoprecipitated using anti-MYC antibody and the immunoprecipitated complex was probed with anti-CFP antibody and visualized using phycoerythrin-conjugated reporter molecules. One HD-Zip I, Zmhdz3, was used to evaluate interaction with ATHB17Δ113. Nomenclature of HD-Zip II and HD-Zip I constructs is based on Zhao et al.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094238#pone.0094238-Zhao1" target="_blank">[22]</a>.</p> 2014-04-15 04:30:42 agriculture Agricultural biotechnology Genetically modified organisms Transgenic plants crops Cereal crops maize biotechnology Genetic engineering Transgenic engineering Plant biotechnology cell biology Molecular cell biology genetics gene expression Plant genetics Plant science forms homodimers makes heterodimers hd-zip ii