ATHB17Δ113 forms homodimers and makes heterodimers with maize HD-Zip II proteins.
Elena A. Rice
Abha Khandelwal
Robert A. Creelman
Cara Griffith
Jeffrey E. Ahrens
J. Philip Taylor
Lesley R. Murphy
Siva Manjunath
Rebecca L. Thompson
Matthew J. Lingard
Stephanie L. Back
Huachun Larue
Bonnie R. Brayton
Amanda J. Burek
Shiv Tiwari
Luc Adam
James A. Morrell
Rico A. Caldo
Qing Huai
Jean-Louis K. Kouadio
Rosemarie Kuehn
Anagha M. Sant
William J. Wingbermuehle
Rodrigo Sala
Matt Foster
Josh D. Kinser
Radha Mohanty
Dongming Jiang
Todd E. Ziegler
Mingya G. Huang
Saritha V. Kuriakose
Kyle Skottke
Peter P. Repetti
T. Lynne Reuber
Thomas G. Ruff
Marie E. Petracek
Paul J. Loida
10.1371/journal.pone.0094238.g006
https://plos.figshare.com/articles/figure/_ATHB17_916_113_forms_homodimers_and_makes_heterodimers_with_maize_HD_Zip_II_proteins_/1000552
<p>Maize leaf protoplasts were (A) mock- transformed or transformed with constructs expressing CFP, MYC::HA and CFP tagged ATHB17Δ113 alone or CFP and ATHB17Δ113::CFP in combination with ATHB17Δ113::MYC construct, (B) transformed (filled bars) with constructs expressing CFP or each of the CFP tagged HD-Zip IIs alone or co-transformed (empty bars) with the construct expressing ATHB17Δ113::MYC-HA. Cellular extracts were co-immunoprecipitated using anti-MYC antibody and the immunoprecipitated complex was probed with anti-CFP antibody and visualized using phycoerythrin-conjugated reporter molecules. One HD-Zip I, Zmhdz3, was used to evaluate interaction with ATHB17Δ113. Nomenclature of HD-Zip II and HD-Zip I constructs is based on Zhao et al.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094238#pone.0094238-Zhao1" target="_blank">[22]</a>.</p>
2014-04-15 04:30:42
agriculture
Agricultural biotechnology
Genetically modified organisms
Transgenic plants
crops
Cereal crops
maize
biotechnology
Genetic engineering
Transgenic engineering
Plant biotechnology
cell biology
Molecular cell biology
genetics
gene expression
Plant genetics
Plant science
forms
homodimers
makes
heterodimers
hd-zip
ii